Informatics Publishing Limited

V. G. Kuchake ¹, P. H. Patil ², H. S. Mahajan ², P. V. Ingle ², S. J. Surana ³, M. M. Thakare ³, S. Sudharshini ⁴

Affiliations
1 Department of Clinical Pharmacy, R.C.Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule, Maharashtra - 425405, IN
2 R.C.Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule, Maharashtra - 425405, IN
3 R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule, Maharashtra - 425405, IN
4 Department of Pharmacy Practice, Vaagdevi College of Pharmacy, Hannamkonda, A.P., IN

Abstract

The present study describes development of a sensitive and simple HPTLC method for estimation of gatifloxacin (GTN) in human urine sample. The drug was extracted using chloroform after adjusting the pH of urine to 7.0. Chloroform extract was spotted on silica gel 60 F254 TLC plate and was developed in a mixture of n-butanol /methanol/ammonia (5:1:2, v/v/v) as the mobile phase and scanned at 292 nm. The peak for GTN resolved at RF of 0.47. The method was validated in terms of linearity (200 to 1200 ng/μL), precision, specificity and accuracy. The limit of detection and limit of quantification for GTN in urine were found to be 25.64 and 77.70 ng, respectively. The average recovery of GTN from urine was 100.68%. The proposed method was applied to generate urinary excretion data for GTN after administration of two market GTN tablet formulations (400 mg, Formulation R and Formulation T) to twelve healthy human volunteers in a two- treatment, open, crossover design. Various pharmacokinetic parameters like peak excretion rate ((dXU/dt)_max), time for peak excretion rate (t_max), AUC_0-48, AUC_0-∞, cumulative amount and % cumulative amount of GTN excreted, elimination half-life (t_1/2), terminal elimination rate constant (k_el) and overall elimination rate constant (K), were calculated for both the formulations. The average cumulative amounts of GTN excreted in urine after administration of Formulation R and Formulation T were found to be 304 ± 11mg (82.32 ± 2.75 % of dose) and 315.2 ± 13.50 mg (78.8 ± 3.37 % of dose), respectively. The urinary excretion profiles of GTN up to 48 h for both the formulations were found to be similar. Statistical comparison (90% confidence intervals of ratio) of various pharmacokinetic parameters of Formulation T with that of Formulation R revealed that Formulation T is bioequivalent with Formulation R.Bioequivalence.

Keywords

Gatifloxacin, HPTLC, Urinary excretion, Bioequivalence.

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H. S. Mahajan ¹, Y. G. Patil ¹, N. A. Hirwe ², T. R. Patil ¹, M. R. Deshmukh ³

Affiliations
1 Oilseed Research Station (MPKV) Jalgaon (M.S.), IN
2 Krishi Vigyan Kendra, Mamurabad Farm, Jalgaon (M.S.), IN
3 Department of Agronomy, Project Co-Ordinating Unit (Sesame and Niger), (JNKVV) Jabalpur (MP), IN

Abstract

Field experiments were conducted on deep black soil of Mamurabad farm, Oilseed Research Station, Jalgaon (Maharashtra). Sesame cv. JLT-7 was sown during 2008,2009 and 2010 to find out suitable combination of soil and foliar application of urea and diammonium phosphate for seed yield maximization and remunerative treatments . Result revealed that, soil application of RDF + foliar spray of 2 per cent urea twice at flowering and pod formation stages significantly improved the yield attributes viz., number of capsules plant -1 and number of seeds capsule-1. These attributes contributed in producing significantly higher seed and oil yields and also proved more remunerative over soil application of RDF alone.

Keywords

Sesame, Foliar Nutrition, Seed Yield, Oil Yield, Economics.

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N. A. Hirwe ¹, H. S. Mahajan ²

Affiliations
1 Department of Agronomy, Krishi Vighan Kendra, Mamurabad Farm, Jalgaon (M.S.), IN
2 Department of Agronomy, Oilseed Research Station, Jalgaon (M.S.), IN

Abstract

The productivity of sesamum is to be quite low due to technological gap in adoption of improved technology. The yield gap between farmers' field and potential yield can effectively bridge by conducting front line demonstrations (FLD) on farmers field. The oilseed Research Station, Jalgaon conducted 31 FLDs during 2007-2011 to analyze impact of improved production technology on farmers field. The result revealed that, sesamum yield increased at an average of 25.44 per cent and received additional net monetary return of Rs. 4066 on ha basis by the adoption of improved production technology.

Keywords

Sesamum, Improved Practices, FLD, Economics.

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N. A. Hirwe ¹, H. S. Mahajan ²

Affiliations
1 Department of Agronomy, Krishi Vighan Kendra, Mamurabad Farm, Jalgaon (M.S.), IN
2 Department of Agronomy, Oilseed Research Station, Jalgaon (M.S.), IN

Abstract

The erratic pattern of rainfall and other climatic situation make it difficult to grown sesamum as sole crop. In order to minimize risk under aberrant weather condition intercropping with pigeon pea is an option for the farmer. The Oilseed Research Station, Jalgaon conducted 12 FLDs during 2007-2011 to observe the performance of sesamum + pigeonpea (4:2) over sole sesamum under real farm situation. The result revealed that, the adoption of sesamum + pigeonpea (4:2) intercropping system increased sesamum equivalent yield by 50.39 per cent and net monetary return by 70.37 per cent over sole sesamum.

Keywords

Intercropping, FLD, Sesamum Equivalent Yield, Economics.

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